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Biosafety cabinet maintenance checklist

Biosafety cabinet maintenance checklist

Stay on top of airflow, filters, and surface integrity with a checklist built for routine biosafety cabinet inspections. It gives you a clear framework to track maintenance steps.

Use this template
or download pdf
Biosafety cabinet maintenance checklist

Stay on top of airflow, filters, and surface integrity with a checklist built for routine biosafety cabinet inspections. It gives you a clear framework to track maintenance steps.

Use this template
or download pdf

About the Biosafety cabinet maintenance checklist

Regular biosafety cabinet checks help prevent cross-contamination and keep staff protected. This biosafety cabinet maintenance checklist gives your team a simple, standardized way to document inspections and stay compliant with safety protocols. With this template, you streamline safety checks, track HEPA filter performance, and improve inspections so that every report is consistent and audit-ready.

How to use the biosafety cabinet maintenance checklist

Whether you’re managing a single lab or coordinating maintenance across multiple facilities, here’s how to make the most of the checklist:

  1. Customize the checklist to match your cabinet model and lab protocols. Start by tailoring the template inside the Lumiform app. Add or remove steps based on the type of cabinet (Class I, II, or III), your lab’s cleaning schedule, and manufacturer requirements. You can also add logic rules to skip steps that don’t apply to your setup.
  2. Assign the checklist to the responsible technician. Once set up, assign it directly to your lab tech or facilities team. They’ll receive it on their mobile device and can complete it digitally, even in areas with low connectivity. 
  3. Complete inspections step by step, with guided input. While they fill out the checklist, they’ll input airflow velocity, pressure readings, and add photos or notes if damage is found. Required fields make sure they don’t miss key details during the process.
  4. Use action tasks to follow up on flagged issues. If the HEPA filter pressure is off or there’s physical damage, create an action task from within the form and track its resolution. This helps you stay on top of repairs without a separate workflow.
  5. Track performance and spot trends with analytics. Over time, your completed checklists will help you identify recurring issues like airflow fluctuations or filter replacements happening too soon. Use the dashboard to catch problems before they disrupt operations.

Best practices for using a biosafety cabinet maintenance checklist

Unlike general lab equipment, a biosafety cabinet has multiple systems working in tandem—airflow, filtration, structural integrity—and each one can quietly fail without obvious signs. 

To prevent this, make it a habit to document readings the same way every time. Use specific units (like m/s for airflow), include exact values rather than approximations, and always photograph visible issues like cracked panels or dislodged gaskets. This builds a reliable maintenance record and gives your team something concrete to review if problems arise.

Don’t delay creating action tasks as well. If airflow is off or filters show signs of stress, trigger a follow-up right from the form. Logging issues without addressing them defeats the point of regular inspections and can lead to downtime or safety risks.

It’s also important to review trends every few months. Recurring low inflow velocity or frequent HEPA replacements might signal a deeper issue with usage or installation. Use Lumiform’s analytics to compare data across cabinets and locations, and loop in your facilities manager or service provider when patterns emerge.

Stay on track with lab safety

Start using this template to build a consistent inspection routine that your team can rely on. With features like smart input fields, image uploads, and built-in action management, you can document issues clearly and respond faster, whether you’re in the lab or reviewing results off-site. Assign it to your staff, collect inspection data in one place, and improve accountability across shifts. 

Related categories

  • Health and safety management templates
  • Health care templates
  • Pharmaceutical industry templates
  • Maintenance templates
  • Safety templates
Preview of the template
Entity
Page 1
Cabinet Exterior
Check for physical damage or cracks on cabinet exterior
Wipe down exterior surfaces with disinfectant
Ensure the work area is clear of clutter and obstructions
Cabinet Interior
Inspect work area for dust, debris or residue
Wipe down interior surfaces with disinfectant
Check for damage or cracks on interior surfaces
Ensure the work tray is clean and positioned correctly
HEPA Filter
Inspect HEPA filter for signs of wear or damage
Check HEPA filter pressure reading, record value
Replace HEPA filter as per manufacturer recommendations
Airflow
Verify cabinet airflow is within specified range
Record inflow velocity reading
Record downflow velocity reading
Documentation
Review and update cabinet certification records
Record any maintenance or repair activities
Ensure all required training is up-to-date

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Related resources

Access a complete set of resources aimed at maximizing safety, quality, and operational excellence, including detailed guides, related templates, and real-world use cases.

Topic guides

Read in-depth guides covering key topics related to this article.

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Frequently asked questions

What should you do before turning on a biosafety cabinet for the day?

Before switching on the cabinet, check that the sash is at the proper working height (usually marked) and that the grille isn’t blocked by papers, tools, or elbows. Let the cabinet run for 5–10 minutes before starting work. This stabilizes airflow and helps clear any residual contaminants from the previous shift.

Is it safe to leave equipment inside the biosafety cabinet overnight?

Not really. since leaving items inside can disrupt airflow, especially if they block rear or front grilles. Over time, this leads to poor containment and increased contamination risk. If you must store something briefly, keep it minimal and push it to the sides without covering vents. Always remove tools and materials after each use.

Can airflow still look normal if the cabinet isn’t functioning properly?

Yes, airflow can appear steady while internal velocities are off, especially if a fan is running but the filter is clogged. This is why visual checks alone aren’t enough. Use an anemometer or airflow monitoring system to confirm inflow and downflow speeds are within safe parameters during regular checks.


This template, developed by Lumiform employees, serves as a starting point for businesses using the Lumiform platform and is intended as a hypothetical example only. It does not replace professional advice. Companies should consult qualified professionals to assess the suitability and legality of using this template in their specific workplace or jurisdiction. Lumiform is not liable for any errors or omissions in this template or for any actions taken based on its content.
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