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Biosafety cabinet preventive maintenance checklist

Biosafety cabinet preventive maintenance checklist

Standardize your biosafety cabinet inspections with this detailed maintenance checklist, from surface checks to airflow testing and documentation tracking.

Use this template
or download pdf
Biosafety cabinet preventive maintenance checklist

Standardize your biosafety cabinet inspections with this detailed maintenance checklist, from surface checks to airflow testing and documentation tracking.

Use this template
or download pdf

About the Biosafety cabinet preventive maintenance checklist

Even the smallest issue in a biosafety cabinet can compromise both product integrity and worker safety, so staying on top of routine checks is critical. This biosafety cabinet preventive maintenance checklist helps your team standardize inspections across labs and sites, from filter integrity to airflow testing. With a clear, repeatable workflow, you’ll spot wear, damage, or contamination risks before they escalate into something much bigger.

Key elements of the biosafety cabinet preventive maintenance checklist

To keep your biosafety cabinets running at peak performance, your maintenance process needs structure. Here’s what the checklist includes:

  1. General cabinet information: Start with the basics: model, manufacturer, serial number, and location. This information ties the checklist to a specific unit and helps you monitor performance or recurring issues over time. It’s especially useful when managing multiple cabinets across sites.
  2. External condition and cleanliness: You can’t overlook the exterior. This section prompts your team to check for physical damage or contamination risks like dust buildup, cracks, or worn panels. Keeping the cabinet clean on the outside reduces the chance of contamination sneaking inside.
  3. Internal components and workspace checks: This is where the daily check happens. Use this part to inspect filters, clean work trays, and check gasket seals. It ensures the internal environment stays sterile and minimizes the risk of airflow disruption or particle buildup.
  4. Airflow performance metrics: Measuring downflow and inflow velocities and comparing them to manufacturer specs lets you confirm the cabinet is still providing safe working conditions. If performance is low, it’s time to recalibrate or replace parts.
  5. Certification and documentation: This section tracks whether certifications are current and whether reports are properly filed. It helps you stay compliant with regulations and ready for audits at any time.

Customizing your biosafety cabinet preventive maintenance checklist 

The frequency and depth of your checks depend on what you’re working with—BSL levels, cabinet class, and how often the unit is used. You can easily adapt the template to your lab’s pace and pressure.

Start by modifying the inspection intervals. If your lab runs 24/7 or handles high-risk agents, you’ll want to set up more frequent checks and additional filter integrity tests. Lumiform lets you duplicate sections or add recurring schedules.

You can also assign specific items to different technician roles. Use logic rules to show or hide tasks based on cabinet class or location. This way, BSL-2 cabinets in a teaching lab won’t get the same checklist as BSL-3 cabinets in a clinical setting.

Lastly, customize the response types. Add photos for visual evidence, or use QR codes to auto-fill cabinet data. This way, you can build a traceable maintenance trail without extra paperwork.

Make inspections faster and more consistent

Download this template and tailor it to your team’s routine in minutes. Assign inspection steps to specific roles, include photo evidence, and track issues in real time. However you run your maintenance, the template adapts without adding extra paperwork, allowing you to focus on compliance and safety.

Related categories

  • Health and safety management templates
  • Health care templates
  • Pharmaceutical industry templates
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  • Safety templates
Preview of the template
Entity
Page 1
General Information
Cabinet Manufacturer
Cabinet Model
Cabinet Serial Number
Date of Inspection
Inspected By
Cabinet Exterior
Clean exterior surfaces
Inspect front view panel for cracks/damage
Inspect side panels for cracks/damage
Inspect rear plenum for dust buildup
Cabinet Interior
Inspect work tray, remove and clean
Inspect interior walls for dust buildup
Inspect supply filter for damage
Inspect exhaust filter for damage
Inspect filter gaskets for proper seal
Cabinet Airflow
Measure downflow velocity (fpm)
Measure inflow velocity (fpm)
Compare values to manufacturer specs
Certification and Documentation
Cabinet certification sticker up-to-date
Certification/Inspection Report on file
Preventive Maintenance Log up-to-date

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Related resources

Access a complete set of resources aimed at maximizing safety, quality, and operational excellence, including detailed guides, related templates, and real-world use cases.

Topic guides

Read in-depth guides covering key topics related to this article.

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See comprehensive collections of best practice templates related to this topic.

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Frequently asked questions

What’s the difference between Class I, II, and III biosafety cabinets when it comes to maintenance?

Each class has unique airflow and containment features, which affects how you maintain them. Class II cabinets, for example, have both inflow and downflow filters, so you’ll need to test both regularly. Class III cabinets are gas-tight and glove-operated, so leak testing and pressure decay checks are a bigger priority than with other types.

Do I need to record maintenance even if nothing’s wrong?

Yes, logging “no issues found” is just as important as documenting repairs. It creates a clear maintenance history, which is useful during audits or if performance starts slipping later on. Plus, it shows that you’re consistently checking, not just reacting when something breaks.

What should I do if airflow readings fall outside the manufacturer’s specs?

Stop work in the cabinet and investigate right away. It could be a clogged pre-filter, damaged HEPA filter, or a fan issue. Document the readings, tag the unit as out-of-service, and schedule servicing. Don’t forget to retest after adjustments to confirm it’s operating safely again.


This template, developed by Lumiform employees, serves as a starting point for businesses using the Lumiform platform and is intended as a hypothetical example only. It does not replace professional advice. Companies should consult qualified professionals to assess the suitability and legality of using this template in their specific workplace or jurisdiction. Lumiform is not liable for any errors or omissions in this template or for any actions taken based on its content.
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